Abstract:
This paper presents a method for prediction of the gradient retention times of six
samples of benzodiazepines – phenazepam, pyrazolam, flubromazepam, meclonazepam,
diclazepam, and diazepam. Isocratic and gradient separation of the compounds of
interest was achieved on a Luna Omega C18 column followed by RP-LC/MS detection.
The results have shown good agreement between the predicted and experimental
retention data; however, higher differences were obtained for fast gradients in the
ultrahigh performance separation mode.
