xmlui.ArtifactBrowser.SimpleSearch.filter.source:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 25/2019
ISSN:1211-5541
Abstract:
This work is focused on the determination of proline in 24 Czech mead samples using
reversed-phase high performance liquid chromatography (RP-HPLC) coupled with
fluorescence detector (operating at excitation wavelength λex = 264 nm and emission
wavelength λem = 497 nm). Sample pre-treatment including solid phase extraction of
mead by Strata SCX SPE column and a derivatization of the extract acquired by dansyl
chloride was performed and thoroughly optimised. Derivative of proline was separated
using Ascentis Express C18 column (150 × 3 mm, 2.7 μm) in the combination with binary
mobile phases that had consisted of 0.1% TEA (pH 3.0) and 100% acetonitrile. The
method proposed could be used for evaluation of mead quality and its authenticity.
