Determination of proline in meads using high performance liquid chromatography

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dc.contributor.author Klikarová, Jitka
dc.contributor.author Šebková, Jana
dc.contributor.author Fischer, Jan
dc.contributor.author Česlová, Lenka
dc.date.accessioned 2019-10-03T12:05:28Z
dc.date.available 2019-10-03T12:05:28Z
dc.date.issued 2019
dc.identifier.isbn 978-80-7560-243-5
dc.identifier.issn 1211-5541
dc.identifier.uri https://hdl.handle.net/10195/74269
dc.description.abstract This work is focused on the determination of proline in 24 Czech mead samples using reversed-phase high performance liquid chromatography (RP-HPLC) coupled with fluorescence detector (operating at excitation wavelength λex = 264 nm and emission wavelength λem = 497 nm). Sample pre-treatment including solid phase extraction of mead by Strata SCX SPE column and a derivatization of the extract acquired by dansyl chloride was performed and thoroughly optimised. Derivative of proline was separated using Ascentis Express C18 column (150 × 3 mm, 2.7 μm) in the combination with binary mobile phases that had consisted of 0.1% TEA (pH 3.0) and 100% acetonitrile. The method proposed could be used for evaluation of mead quality and its authenticity. en
dc.format p. 15 - 26
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 25/2019 en
dc.rights open access en
dc.subject proline en
dc.subject dansyl chloride en
dc.subject meads en
dc.subject derivatization en
dc.subject extraction en
dc.subject liquid chromatography en
dc.title Determination of proline in meads using high performance liquid chromatography en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en


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