xmlui.ArtifactBrowser.SimpleSearch.filter.source:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013
ISSN:1211-5541
Abstract:
A relatively simple method for the simultaneous determination of epinephrine,
norepinephrine and dopamine in urine is presented. We have used a highperformance
liquid chromatography (HPLC) with coulometric electrochemical
detection. The urine samples pretreated by a solid-phase extraction were injected
directly into the HPLC system and were eluted isocratically on a Discovery HS
C18, 250×4 mm i.d., 5 μm, analytical column. The analytical performance of this
method is satisfactory: the intra-assay and inter-assay coefficients of variation
were below 10 %. Quantitative recoveries from spiked urine samples were in the
intervals of 78.2-88.0 % for epinephrine, 80.0-93.0 % for norepinephrine, and
96.4-107.2 % for dopamine. The linear range is 2-200 μg l–1, with a detection limit of 0.6 μg l–1 (signal-to-noise ratio = 3) for epinephrine, 5-500 μg l–1, with a
detection limit of 1.5 μg l–1 for norepinephrine, and 20-2000 μg l–1, with a
detection limit of 6 μg l–1 for dopamine.
