xmlui.ArtifactBrowser.SimpleSearch.filter.source:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 21/2015
ISSN:1211-5541
Abstract:
Herein, a method is described for the determination of malondialdehyde,
formaldehyde, acetaldehyde, and propionaldehyde in human plasma. Plasma
sampleswere obtained fromblood donors. After protein precipitation, the samples
were derivatized with 2,4-dinitrophenylhydrazine and repeatedly extracted with
pentane. So combined pentane extracts were evaporated to dryness, under
nitrogen atmosphere, and the dried residue was re-suspended in acetonitrile.
Aliquots of 20 μl in acetonitrile were injected onto a LichroCART 125-4,
Purospher STAR RP-18e, 5 μm column. The derivatives were analysed with the
aid of gradient elution, when using a mobile phase containing acetonitrile, water,
and acetic acid. The analytical performance of this method has been found to be satisfact ory characterised by the intra-assay coefficients of variation below 12 %
and quantitative recoveries as follows: 98.3 % (CV 1.6%) for malondialdehyde,
104.4 % (CV 4.0 %) for formaldehyde, 96.9 % (CV 3.0 %) for acetaldehyde, and
105.9 % (CV 1.7 %) for propionaldehyde.
