xmlui.ArtifactBrowser.SimpleSearch.filter.source:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 24/2018
ISSN:1211-5541
Abstract:
This work deals with the study of lipid metabolism in type 2 diabetics focusing on the
changes in odd-numbered fatty acid concentrations. Diabetes mellitus is a syndrome of
metabolic disorders in which the concentration of insulin and its secretion are impaired.
It indicates an impaired metabolism of glucose, lipids, and proteins. The main goal of
this work was to measure concentrations of odd-numbered fatty acids (ONFA) in
healthy individuals and in patients with type 2 diabetes mellitus and to compare the
concentrations between both groups by using statistical methods. Plasma samples were
deproteinized and lipids were extracted into organic solvents. Thin layer
chromatography was used to separate 5 lipid fractions. All the lipid fractions were
derivatized into fatty acid methyl esters and analyzed by a gas chromatograph. ONFA
are minor group of the fatty acids and their concentrations are lower in type 2 diabetes
mellitus. The most significant changes in concentrations were registered for tridecanoic
and pentadecanoic acid.
