Multichannel separation device with parallel electrochemical detection
Článekpeer-reviewedpublished versionDatum publikování
2020
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Elsevier Science BV
Abstrakt
A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.
Rozsah stran
p. 460537
ISSN
0021-9673
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Zdrojový dokument
Journal of Chromatography A, volume 1610, issue: January
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https://www.sciencedirect.com/science/article/pii/S0021967319309306
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pouze v rámci univerzity
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Klíčová slova
3D printing, multi-channel device, dopamine metabolism, electrochemical detection, polymer monoliths, titanium, 3D tisk, vícekanálové zařízení, metabolismus dopaminu, elektrochemická detekce, polymerní monolity, titan