Zobrazit minimální záznam
dc.contributor.author |
Drábková, Petra
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dc.contributor.author |
Bencová, Soňa
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|
dc.contributor.author |
Štramová, Xenie
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dc.contributor.author |
Žáková, Pavla
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dc.contributor.author |
Kanďár, Roman
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dc.date.accessioned |
2020-06-10T09:05:49Z |
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dc.date.available |
2020-06-10T09:05:49Z |
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dc.date.issued |
2013 |
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dc.identifier.isbn |
978-80-7395-748-3 |
|
dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/75465 |
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dc.description.abstract |
A relatively simple method for the simultaneous determination of epinephrine,
norepinephrine and dopamine in urine is presented. We have used a highperformance
liquid chromatography (HPLC) with coulometric electrochemical
detection. The urine samples pretreated by a solid-phase extraction were injected
directly into the HPLC system and were eluted isocratically on a Discovery HS
C18, 250×4 mm i.d., 5 μm, analytical column. The analytical performance of this
method is satisfactory: the intra-assay and inter-assay coefficients of variation
were below 10 %. Quantitative recoveries from spiked urine samples were in the
intervals of 78.2-88.0 % for epinephrine, 80.0-93.0 % for norepinephrine, and
96.4-107.2 % for dopamine. The linear range is 2-200 μg l–1, with a detection limit of 0.6 μg l–1 (signal-to-noise ratio = 3) for epinephrine, 5-500 μg l–1, with a
detection limit of 1.5 μg l–1 for norepinephrine, and 20-2000 μg l–1, with a
detection limit of 6 μg l–1 for dopamine. |
en |
dc.format |
p. 25–38 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013 |
en |
dc.rights |
open access |
en |
dc.title |
Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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