An assay of creatine in human serum using an HPLC with UV detection

Show simple item record Štramová, Xenie Drábková, Petra Kanďár, Roman 2020-06-10T08:40:03Z 2020-06-10T08:40:03Z 2013
dc.identifier.isbn 978-80-7395-748-3
dc.identifier.issn 1211-5541
dc.description.abstract An ion-exchange high-performance liquid chromatography with ultraviolet detection for the determination of creatine in human serum has been developed. The human serum was deproteinized with perchloric acid. For the separation, a cation-exchange column LC-SCX, 250 mm × 4.6 mm, 5 μm, was used. The mixture of ethanol and 10 mmol l–1 ammonium acetate (15:85, v/v), pH 4.00 ± 0.05 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries of spiked serum samples were between 97.1 % and 103.5 %. The calibration curve was linear in the whole range tested. The limit of quantification was 2 μmol l–1. The preliminary reference ranges of serum creatine in a group of blood donors are 17-89 μmol l–1 for women and 21-97 μmol l–1 for men. en
dc.format p. 39–48
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013 en
dc.rights open access en
dc.title An assay of creatine in human serum using an HPLC with UV detection en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en

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