Utilization of hydrosilated silica-based stationary phases for separation of antioxidants

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dc.contributor.author Soukup, Jan
dc.contributor.author Janás, Petr
dc.contributor.author Jandera, Pavel
dc.date.accessioned 2020-05-20T09:15:24Z
dc.date.available 2020-05-20T09:15:24Z
dc.date.issued 2014
dc.identifier.isbn 978-80-7395-814-5
dc.identifier.issn 1211-5541
dc.identifier.uri https://hdl.handle.net/10195/75432
dc.description.abstract We studied the influence of mobile phase composition on the retention of phenolic acids and flavonoid compounds on five different hydrosilated silica-based stationary phases in buffered aqueous acetonitrile. Cogent UDC cholesterolTM, Cogent bidentate C18TM and Cogent Phenyl hydrideTM columns show significant dual reversed-phase/normal-phase retention behaviour, while Cogent Diamond hydrideTM and Cogent Silica-CTM columns provide very low retention in the reversed-phase mode. The effect of the aqueous acetate buffer concentration on retention factors of phenolic acids and flavonoid compounds over the full mobile phase composition range, including both aqueous normal-phase (ANP) and reversed-phase (RP) mechanisms, can be described by a four-parameter equation for dual-retention mechanism. At increasing temperature, the retention factors and peak widths decrease in the aqueous normal-phase range. In agreement with van’t Hoff model, linear ln k versus 1/T plots were observed, showing a single retention mechanism. From among the stationary phases tested, Cogent UDC cholesterolTM column has high temperature stability (up to 100 °C) and provides the most selective and efficient separations of flavones in the ANP mode and Cogent Diamond hydrideTM is the most selective and effective for separation of phenolic acids. en
dc.format p. 37–70
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 20/2014 en
dc.rights open access en
dc.title Utilization of hydrosilated silica-based stationary phases for separation of antioxidants en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en

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