Digitální knihovnaUPCE
 

An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high-performance liquid chromatography with fluorescence detection

Článekpeer-reviewedpublished
Náhled

Datum publikování

2012

Vedoucí práce

Oponent

Název časopisu

Název svazku

Vydavatel

University of Pardubice

Abstrakt

A method is described for the determination of 4-hydroxy-trans-2-nonenal in human seminal plasma. Semen samples were obtained from male partners of couples presenting for a fertility evaluation. After liquefaction, the samples were centrifuged and the seminal plasma was stored at –80 °C. 4-Hydroxy-trans-2- nonenal was derivatized with 1,3-cyclohexanedione to generate the fluorescent alkyl acridine derivative. After derivatization, seminal plasma proteins were precipitated with cold perchloric acid, and the supernatant was injected into the HPLC system. The separation was realized on an analytical reversed-phase column with fluorescence detection. The mixture of ethanol and deionized water was used as the mobile phase. The intra-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked seminal plasma were between 62.0 and 81.0 %.

Rozsah stran

p. 17–28

ISSN

1211-5541

Trvalý odkaz na tento záznam

Projekt

Zdrojový dokument

Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 18/2012

Vydavatelská verze

Přístup k e-verzi

open access

Název akce

ISBN

978-80-7395-577-9

Studijní obor

Studijní program

Signatura tištěné verze

Umístění tištěné verze

Přístup k tištěné verzi

Klíčová slova

Endorsement

Review

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