Biofilm production by Staphylococcus aureus strains isolated from cystic fibrosin patients
ČlánekOtevřený přístuppeer-reviewedpublishedSoubory
Datum publikování
2012
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
University of Pardubice
Abstrakt
Cystic fibrosis is a serious genetic disorder affecting respiratory, gastrointestinal
and genital tracts. The most common causes of health status deterioration of
patients with cystic fibrosis are still considered to be bacterial infections.
Staphylococcus aureus is one of the most common pathogens isolated from the air
passages of these patients. The purpose of our study was to determine the ability
and intensity of the biofilm production by 59 of Staphylococcus aureus strains
isolated from the air passages of patients with cystic fibrosis in the Cystic fibrosis
center at the University Hospital Hradec Kralové. We also evaluated biofilm
production and its intensity in 59 Staphylococcus aureus strains isolated from the
air passages of control group patients. The control group was formed by patients
with nosocomial pneumonia hospitalized in the University Hospital. For assessing the biofilm production, we used modified microtiter-plate test by Christensen. We
divided the intensity of biofilm production into four categories according to the
measured optical density — negative, weak, medium and strong. Out of total 59
Staphylococcus aureus strains isolated from the air passages of cystic fibrosis
patients 45 (77.2 %) strains produced biofilm in various degrees: 21 (35.6 %)
weakly, 6 (10.2 %) medium and 18 (30.5 %) strongly. Similar results were
determined for strains isolated from control group: 22 (37.3 %) weak, 13 (22.0 %)
medium and 9 (15.3 %) strong biofilm producing strains. We found no statistically
significant difference in the biofilm formation between both groups of
Staphylococcus aureus strains.
Rozsah stran
p. 37–46
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 18/2012
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
978-80-7395-577-9