Show simple item record
dc.contributor.author |
Štramová, Xenie
|
|
dc.contributor.author |
Kanďár, Roman
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dc.date.accessioned |
2020-05-11T17:10:00Z |
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dc.date.available |
2020-05-11T17:10:00Z |
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dc.date.issued |
2011 |
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dc.identifier.isbn |
978-80-7395-459-8 |
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dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/75365 |
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dc.description.abstract |
A reversed-phase high-performance liquid chromatography method for the
determination of creatinine in human serum samples has been developed. Results
from measurements of by a high-performance liquid chromatography on 232
serum samples were compared with enzymatic and Jaffé methods. The serum
samples were deproteinized with ethanol. For the separation, a column MAG 1,
250 mm x 4.6 mm, Labiospher PSI 100 C18, 5 :m, was used. The mixture of
ethanol and 25 mmol l–1 sodium hydrogenphosphate (3:97 v/v), pH 6.5 was used
as a mobile phase. The analytical performance of this method is satisfactory: the
intra-assay and inter-assay coefficients of variation were below 6 %. Quantitative
recoveries of spiked serum samples were between 101.8 and 106.0 %. The limit of
quantification was 10.0 :mol l–1. The results obtained by chromatographic method
correlated well with Jaffé assays. Enzymatic method gave at average significantly
higher values depending on a group of patients. The presented high-performance liquid chromatographic assay is useful for the analysis of samples where the
classical Jaffé or enzymatic methods do not give reliable results and can be a
candidate for reference method. |
en |
dc.format |
p. 57–70 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 17/2011 |
en |
dc.rights |
open access |
en |
dc.title |
Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection – comparison with Jaffé and enzymatic assays |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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