Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection – comparison with Jaffé and enzymatic assays

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dc.contributor.author Štramová, Xenie
dc.contributor.author Kanďár, Roman
dc.date.accessioned 2020-05-11T17:10:00Z
dc.date.available 2020-05-11T17:10:00Z
dc.date.issued 2011
dc.identifier.isbn 978-80-7395-459-8
dc.identifier.issn 1211-5541
dc.identifier.uri https://hdl.handle.net/10195/75365
dc.description.abstract A reversed-phase high-performance liquid chromatography method for the determination of creatinine in human serum samples has been developed. Results from measurements of by a high-performance liquid chromatography on 232 serum samples were compared with enzymatic and Jaffé methods. The serum samples were deproteinized with ethanol. For the separation, a column MAG 1, 250 mm x 4.6 mm, Labiospher PSI 100 C18, 5 :m, was used. The mixture of ethanol and 25 mmol l–1 sodium hydrogenphosphate (3:97 v/v), pH 6.5 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 6 %. Quantitative recoveries of spiked serum samples were between 101.8 and 106.0 %. The limit of quantification was 10.0 :mol l–1. The results obtained by chromatographic method correlated well with Jaffé assays. Enzymatic method gave at average significantly higher values depending on a group of patients. The presented high-performance liquid chromatographic assay is useful for the analysis of samples where the classical Jaffé or enzymatic methods do not give reliable results and can be a candidate for reference method. en
dc.format p. 57–70
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 17/2011 en
dc.rights open access en
dc.title Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection – comparison with Jaffé and enzymatic assays en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en


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