Digitální knihovnaUPCE
 

Catalitic activity of Agaricus bisporus mushroom tyrosinase to acetaminophenon in the presence of L-proline

ČlánekStatus neznámýpeer-reviewedpublisher's version
Náhled

Datum publikování

2017

Vedoucí práce

Oponent

Název časopisu

Název svazku

Vydavatel

University of Pardubice

Abstrakt

Secondary reaction of amino acid L-proline with 4-acetamido-o-benzoquinone as a product of the enzymatic oxidation of acetaminophen mediated by mushroom tyrosinase isolated from Agaricus bisporus has been studied by amperometric detection in the batch injection analysis configuration and by ultraviolet-visible spectrophotometry. Actually, this scientific work is focused on finding out whether it would be possible to develop an indirect electrochemical method based on the amperometric biosensing suitable for monitoring of the L-proline in various honey samples. For optimum content of acetaminophen (100 :mol dm–3) and tyrosinase (3.33 :g cm–3), a satisfactory linear range from 0.1 to 0.5 mmol dm–3 L-proline was obtained for absorption maximum at 332 nm. However, it is necessary to mention that the indirect determination of L-proline by the amperometric tyrosinase biosensor still presents a challenge in the field of developing biological sensors for monitoring of this amino acid.

Rozsah stran

p. 85–97

ISSN

1211-5541

Trvalý odkaz na tento záznam

Projekt

Zdrojový dokument

Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 23/2017

Vydavatelská verze

Přístup k e-verzi

open access

Název akce

ISBN

978-80-7560-090-5

Studijní obor

Studijní program

Signatura tištěné verze

Umístění tištěné verze

Přístup k tištěné verzi

Klíčová slova

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Review

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