Electrochemical detection of 8-hydroxyguanine using screen-printed carbon electrodes modified with carboxy-functionalized multi-walled carbon nanotubes
ČlánekOmezený přístuppeer-reviewedpublished versionDatum publikování
2019
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Springer
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The paper presents the findings of an optimization and validation results of electrochemical detection of 8-hydroxyguanine, a major marker of oxidative stress, using disposable screen-printed carbon electrode (SPCE) modified with carboxy-functionalized multi-walled carbon nanotubes (MWCNTs-COOH). Several types of bare and modified SPCEs were tested and modification with MWCNTs-COOH was found superior in terms of measured current response, level of background currents, and sensitivity in oxidation of 8-hydroxyguanine. The effect of pH and composition of supporting electrolyte on current response and peak potential were also evaluated. Validation parameters were calculated for optimum pH 7 of 0.01moldm(-3) phosphate buffer. The sensor showed the highest sensitivity of 0.0625 mu A dm(3) mu mol(-1) within the linear range 0.3-12 mu moldm(-3) with a limit of detection as low as 0.57 mu moldm(-3). Extended linear range 0.3-240 mu moldm(-3) is feasible with a sensitivity of 0.0523 mu A dm(3) mu mol(-1). Intra-day and inter-day assays within 3days were performed to verify the repeatability of modified screen-printed carbon sensors. Finally, the sensor was utilized for electrochemical monitoring of stability of 8-hydroxyguanine when stored in room temperature, in the refrigerator, or frozen and thawed. [GRAPHICS] .
Rozsah stran
p. 1187-1193
ISSN
0026-9247
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Monatshefte fur Chemie, volume 150, issue: 7
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https://link.springer.com/article/10.1007/s00706-019-02433-3
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8-Hydroxyguanine, Screen-printed carbon electrode, Multi-walled carbon nanotubes, Differential pulse voltammetry, 8-Hydroxyguanin, Uhlíková tištěná elektroda, Vícestěnné uhlíkové nanotrubičky, Diferenční pulzní voltametrie