Determination of proline in meads using high performance liquid chromatography
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2019
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
University of Pardubice
Abstrakt
This work is focused on the determination of proline in 24 Czech mead samples using
reversed-phase high performance liquid chromatography (RP-HPLC) coupled with
fluorescence detector (operating at excitation wavelength λex = 264 nm and emission
wavelength λem = 497 nm). Sample pre-treatment including solid phase extraction of
mead by Strata SCX SPE column and a derivatization of the extract acquired by dansyl
chloride was performed and thoroughly optimised. Derivative of proline was separated
using Ascentis Express C18 column (150 × 3 mm, 2.7 μm) in the combination with binary
mobile phases that had consisted of 0.1% TEA (pH 3.0) and 100% acetonitrile. The
method proposed could be used for evaluation of mead quality and its authenticity.
Rozsah stran
p. 15 - 26
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 25/2019
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
978-80-7560-243-5
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
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Klíčová slova
proline, dansyl chloride, meads, derivatization, extraction, liquid chromatography