Covalent biofunctionalization of chitosan nanofibers with trypsin for high enzyme stability

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dc.contributor.author Slováková, Marcela
dc.contributor.author Srbová, Jana
dc.contributor.author Křípalová, Zuzana
dc.contributor.author Žárská, Monika
dc.contributor.author Špačková, Martina
dc.contributor.author Stránská, Denisa
dc.contributor.author Bílková, Zuzana
dc.date.accessioned 2019-08-14T08:23:44Z
dc.date.available 2019-08-14T08:23:44Z
dc.date.issued 2016
dc.identifier.issn 1381-5148
dc.identifier.uri https://hdl.handle.net/10195/73811
dc.description.abstract The electrospun chitosan nanofibers provide excellent material for immobilized proteolytic enzymes, and are biocompatible, nontoxic and hydrophilic matrices with large specific area. This paper deals with an application of electrospun chitosan nanofibers and optimizing conditions for their biofunctionalization by model proteolytic enzyme trypsin. Nanofibers fromchitosan were prepared using Nanospider™ technology and covalent immobilization of trypsin followed. Three immobilization techniques preserving biocompatibility and utilizing amine and/or hydroxyl groups of chitosan were optimized and compared to simple adsorption to achieve maximum proteolytic activity per cm2 of the functionalized chitosan nanofibers (Tryp-NF). Significant differences were observed. Trypsin immobilized by the carbodiimide one-step protocol demonstrated the highest activity of the three procedures, ranging from 132 to 210 IU/cm2 (i.e., 548–874 IU/mg of nanofibers), depending on the initial amount of trypsin used. Long-term storage stability together with high reusability of Tryp-NF confirmed advantages of the immobilized enzyme. Tryp-NF showed no cytotoxicity toward growth of HeLa cells. The in vivo tests for irritation and skin sensitization demonstrated no undesirable skin reactions. eng
dc.format p. 38–44 eng
dc.language.iso eng cze
dc.publisher Elsevier Science BV cze
dc.relation.ispartof Reactive and Functional Polymers 104 (2016) eng
dc.subject trypsin eng
dc.subject chitosan eng
dc.subject electrospun nanofibers eng
dc.subject reusability eng
dc.subject biofunctionalization eng
dc.title Covalent biofunctionalization of chitosan nanofibers with trypsin for high enzyme stability eng
dc.title.alternative Kovalentní biofunkcionalizace chitosanových nanovláken s trypsinem s vysokou enzymovou stabilitou cze
dc.type article eng
dc.description.abstract-translated Kovalentní biofunkcionalizace chitosanových nanovláken s trypsinem s vysokou enzymovou stabilitou. Elektrozvlákněná chitosanová nanovlákna slouží jako výborný materiál pro imobilizaci proteolytických enzymů, jsou biokompatibilní, netoxické a hydrofilní matricí s velkým specifickým povrchem. cze
dc.peerreviewed yes eng
dc.publicationstatus postprint eng
dc.identifier.doi 10.1016/j.reactfunctpolym.2016.05.009
dc.identifier.wos 000379375400006
dc.identifier.scopus 2-s2.0-84969577643
dc.identifier.obd 39876684


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