Covalent biofunctionalization of chitosan nanofibers with trypsin for high enzyme stability
ČlánekOtevřený přístuppeer-reviewedpostprintDatum publikování
2016
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Elsevier Science BV
Abstrakt
The electrospun chitosan nanofibers provide excellent material for immobilized proteolytic enzymes, and are
biocompatible, nontoxic and hydrophilic matrices with large specific area. This paper deals with an application
of electrospun chitosan nanofibers and optimizing conditions for their biofunctionalization by model proteolytic
enzyme trypsin. Nanofibers fromchitosan were prepared using Nanospider™ technology and covalent immobilization
of trypsin followed. Three immobilization techniques preserving biocompatibility and utilizing amine
and/or hydroxyl groups of chitosan were optimized and compared to simple adsorption to achieve maximum
proteolytic activity per cm2 of the functionalized chitosan nanofibers (Tryp-NF). Significant differences were observed.
Trypsin immobilized by the carbodiimide one-step protocol demonstrated the highest activity of the
three procedures, ranging from 132 to 210 IU/cm2 (i.e., 548–874 IU/mg of nanofibers), depending on the initial
amount of trypsin used. Long-term storage stability together with high reusability of Tryp-NF confirmed advantages
of the immobilized enzyme. Tryp-NF showed no cytotoxicity toward growth of HeLa cells. The in vivo tests
for irritation and skin sensitization demonstrated no undesirable skin reactions.
Rozsah stran
p. 38–44
ISSN
1381-5148
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Reactive and Functional Polymers 104 (2016)
Vydavatelská verze
Přístup k e-verzi
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
trypsin, chitosan, electrospun nanofibers, reusability, biofunctionalization