Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples
ČlánekOmezený přístuppeer-reviewedpublishedDatum publikování
2018
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Abstrakt
The fast and efficient detection of foodborne pathogens is a societal priority, given the large number of food-poisoning outbreaks, and a scientific and technological challenge, given the need to detect as little as 1 viablecell in 25 gr of food. Here, we present thefirst approach that achieves the above goal, thanks to the use of amicro/nano-technology and the detection capability of acoustic wave sensors. Starting from 1Salmonellacell in25 ml of milk, we employ immuno-magnetic beads to capture cells after only 3 h of pre-enrichment and sub-sequently demonstrate efficient DNA amplification using the Loop Mediated Isothermal Amplification method(LAMP) and acoustic detection in an integrated platform, within an additional ½ h. The demonstrated 4 hsample-to-analysis time comes as a huge improvement to the current need of few days to obtain the same result.In addition, the work presents thefirst reported Lab-on-Chip platform that comprises an acoustic device as thesensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μlor3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples. The useof food samples together with the incorporation of the necessary pre-enrichment step and ability for multipleanalysis with an internal control, make the proposed methodology highly relevant to real-world applications.Moreover, the work suggests that acoustic wave devices can be used as an attractive alternative to electro-chemical sensors in integrated platforms for applications in food safety and the point-of-care diagnostics.
Rozsah stran
p. 52-58
ISSN
0956-5663
Trvalý odkaz na tento záznam
Projekt
687681/A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument (LoveFood2Market)
Zdrojový dokument
Biosensors and Bioelectronics, volume 111, issue: 15.7. 2018
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pouze v rámci univerzity
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Klíčová slova
Lab-on-chip, Molecular diagnostics, Acoustic biosenzor, Salmonella detection, Lab-on-chip, Molekulární diagnostika, akustický biosenzor, detekce Salmonelly