Inhibition of acetylcholinesterase and butyrylcholinesterase by a plant secondary metabolite boldine

Show simple item record Kostelník, Adam cze Pohanka, Miroslav cze 2019-05-22T08:09:56Z 2019-05-22T08:09:56Z 2018 eng
dc.identifier.issn 2314-6133 eng
dc.description.abstract Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are two enzymes sensitive to various chemical compounds having ability to bind to crucial parts of these enzymes. Boldine is a natural alkaloid and it was mentioned in some older works that it can inhibit some kinds ofAChE.We reinvestigated this effect onAChE and also on BChE using acetyl (butyryl) thiocholine and Ellman’s reagents as standard substances for spectrophotometric assay.We found out IC50 of AChE equal to 372 𝜇mol/l and a similar level to BChE, 321 𝜇mol/l.We conclude our experiment by a finding that boldine is cholinesterase inhibitor; however we report significantly weaker inhibition than that suggested in literature. Likewise, we tried to investigate the mechanism of inhibition and completed it with in silico study. Potential toxic effect on cholinesterases in real conditions is also discussed. eng
dc.format p. "9634349-1"-"9634349-5" eng
dc.language.iso eng eng
dc.relation.ispartof Biomed Research International, volume Neuveden, issue: 2018 eng
dc.rights open access eng
dc.subject Acetylcholinesterase eng
dc.subject butyrylcholinesterase eng
dc.subject inhibition eng
dc.subject docking eng
dc.subject boldine eng
dc.subject Acetylcholinesterasa cze
dc.subject butyrylcholinesterasa cze
dc.subject inhibice cze
dc.subject dokování cze
dc.subject boldin cze
dc.title Inhibition of acetylcholinesterase and butyrylcholinesterase by a plant secondary metabolite boldine eng
dc.title.alternative Inhibiční studie boldinu na acetylcholinesterasu a butyrylcholinesterasu cze
dc.type article eng
dc.description.abstract-translated Acetylcholinesterasa (AChE) and butyrylcholinesterasa (BChE) jsou enzymy citlivé k rozličným inhibitorům. Boldin je přírodní alkaloid, který byl v literatuře zmíněn jako inhibitor AChE. V naší práci byl přezkoumán jeho efekt na AChE a rovněž BChE za použití standardní Ellmanovy metody. Z výsledků byly vypočteny IC50 pro AChE (372 𝜇mol/l) a pro BChE (321 𝜇mol/l). Rovněž bylo provedeno modelování boldinu do struktury cholinesteras pro zjištění mechanismu molekulární interakce. cze
dc.peerreviewed yes eng
dc.publicationstatus published eng
dc.identifier.doi 10.1155/2018/9634349 eng
dc.relation.publisherversion eng
dc.identifier.wos 000429260400001
dc.identifier.scopus 2-s2.0-85045755692
dc.identifier.obd 39880830 eng

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