Digitální knihovnaUPCE
 

Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxic compound assay by a photographic technique

Článekpeer-reviewedpublished
Náhled

Datum publikování

2018

Vedoucí práce

Oponent

Název časopisu

Název svazku

Vydavatel

Abstrakt

Smartphones scored big success in the last decade and they broke into analytical chemistry as an easy detection system with comparable parameters to standard laboratory equipment. This work focuses on attachment of enzyme acetylcholinesterase (AChE) onto previously activated chitosan. Here, AChE splits alternative substrate indoxyl acetate. Conversion of substrate is blocking or slowing down by inhibitors from which galantamine and tacrine were tested here as model inhibitors with detection limits, 1.1 µM and 0.18 µM, respectively. Measurement procedure was carried out on a 3D three-dimensional printed holder and red-green-blue (RGB) channels served for digital photography evaluation. The method was validated to standard Ellman´s spectrophotometric assay. Long-term stability of immobilized enzyme as well as sensitivity to organic solvents were also tested.

Rozsah stran

p. 1622-1632

ISSN

0003-2719

Trvalý odkaz na tento záznam

Projekt

Zdrojový dokument

Analytical Letters, volume 51, issue: 10

Vydavatelská verze

http://www.tandfonline.com/doi/abs/10.1080/00032719.2017.1381846

Přístup k e-verzi

Pouze v rámci UPa

Název akce

ISBN

Studijní obor

Studijní program

Signatura tištěné verze

Umístění tištěné verze

Přístup k tištěné verzi

Klíčová slova

Acetylcholinesterase, chitosan, indoxylacetate, immobilization, Acetylcholinesteráza, chitosan, indoxylacetát, imobilizace

Endorsement

Review

item.page.supplemented

item.page.referenced