Zobrazit minimální záznam
dc.contributor.author |
Hauschke, Martina |
cze |
dc.contributor.author |
Roušarová, Erika
|
cze |
dc.contributor.author |
Flidr, Pavel |
cze |
dc.contributor.author |
Čapek, Jan
|
cze |
dc.contributor.author |
Libra, Antonín |
cze |
dc.contributor.author |
Roušar, Tomáš
|
cze |
dc.date.accessioned |
2018-02-27T03:42:38Z |
|
dc.date.available |
2018-02-27T03:42:38Z |
|
dc.date.issued |
2017 |
eng |
dc.identifier.issn |
0887-2333 |
eng |
dc.identifier.uri |
https://hdl.handle.net/10195/70300 |
|
dc.description.abstract |
Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity. HK-2 cells were incubated with selected compounds for 1-24h and cell viability was measured together with extracellular NGAL production. We proved that HK-2 cells possess a capacity to produce NGAL in amount of 2pg/ml/h. We found a change in cell viability after 24h incubation with all tested toxic compounds. The largest decrease of the viability was detected in mercury, acetaminophen, cisplatin and gentamicin. Unexpectedly, we found also a significant decrease in NGAL production in HK-2 cells treated with these toxins for 24h: to 11±5%, 54±5%, 57±6% and 76±9% respectively, compared with controls (=100%). Our results were followed with qPCR analysis when we found no significant increase in LCN2 gene expression after 24h incubation. We conclude that extracellular NGAL production negatively correlates with HK-2 cell impairment. |
eng |
dc.format |
p. 52-57 |
eng |
dc.language.iso |
eng |
eng |
dc.relation.ispartof |
Toxicology in Vitro, volume 39, issue: Mar |
eng |
dc.rights |
Práce není přístupná |
eng |
dc.subject |
Cell viability |
eng |
dc.subject |
HK-2 cells |
eng |
dc.subject |
In vitro nephrotoxicity |
eng |
dc.subject |
NGAL |
eng |
dc.subject |
Nephrotoxicity markers |
eng |
dc.subject |
Viabilita buněk |
cze |
dc.subject |
HK-2 buňky |
cze |
dc.subject |
in vitro neforotoxicita |
cze |
dc.subject |
NGAL |
cze |
dc.subject |
markery nefrotoxicity |
cze |
dc.title |
Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells |
eng |
dc.title.alternative |
NGAL produkce negativně koreluje s poškozením HK-2 buněk. |
cze |
dc.type |
article |
eng |
dc.description.abstract-translated |
NGAL produkce negativně koreluje s poškozením HK-2 buněk. NGAL je extracelulární protein produkovaný převážně v ledvinách. Nedávno byl použit jako slibný biomarker pro detekci renálního poškození in vivo. Přesto dosud nebyl použit jako biomarker in vitro, a ověření tohoto bylo hlavním cílem naší studie. |
cze |
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
published |
eng |
dc.identifier.doi |
10.1016/j.tiv.2016.11.012 |
eng |
dc.relation.publisherversion |
http://www.sciencedirect.com/science/article/pii/S0887233316302430/pdfft?md5=ffce0ffeb7593cedc9fd3da7b17225cc&pid=1-s2.0-S0887233316302430-main.pdf |
eng |
dc.identifier.wos |
000393542600004 |
eng |
dc.identifier.scopus |
2-s2.0-84997783020 |
|
dc.identifier.obd |
39879452 |
eng |
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