Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells

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dc.contributor.author Hauschke, Martina cze
dc.contributor.author Roušarová, Erika cze
dc.contributor.author Flidr, Pavel cze
dc.contributor.author Čapek, Jan cze
dc.contributor.author Libra, Antonín cze
dc.contributor.author Roušar, Tomáš cze
dc.date.accessioned 2018-02-27T03:42:38Z
dc.date.available 2018-02-27T03:42:38Z
dc.date.issued 2017 eng
dc.identifier.issn 0887-2333 eng
dc.identifier.uri https://hdl.handle.net/10195/70300
dc.description.abstract Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity. HK-2 cells were incubated with selected compounds for 1-24h and cell viability was measured together with extracellular NGAL production. We proved that HK-2 cells possess a capacity to produce NGAL in amount of 2pg/ml/h. We found a change in cell viability after 24h incubation with all tested toxic compounds. The largest decrease of the viability was detected in mercury, acetaminophen, cisplatin and gentamicin. Unexpectedly, we found also a significant decrease in NGAL production in HK-2 cells treated with these toxins for 24h: to 11±5%, 54±5%, 57±6% and 76±9% respectively, compared with controls (=100%). Our results were followed with qPCR analysis when we found no significant increase in LCN2 gene expression after 24h incubation. We conclude that extracellular NGAL production negatively correlates with HK-2 cell impairment. eng
dc.format p. 52-57 eng
dc.language.iso eng eng
dc.relation.ispartof Toxicology in Vitro, volume 39, issue: Mar eng
dc.rights Práce není přístupná eng
dc.subject Cell viability eng
dc.subject HK-2 cells eng
dc.subject In vitro nephrotoxicity eng
dc.subject NGAL eng
dc.subject Nephrotoxicity markers eng
dc.subject Viabilita buněk cze
dc.subject HK-2 buňky cze
dc.subject in vitro neforotoxicita cze
dc.subject NGAL cze
dc.subject markery nefrotoxicity cze
dc.title Neutrophil gelatinase-associated lipocalin production negatively correlates with HK-2 cell impairment: Evaluation of NGAL as a marker of toxicity in HK-2 cells eng
dc.title.alternative NGAL produkce negativně koreluje s poškozením HK-2 buněk. cze
dc.type article eng
dc.description.abstract-translated NGAL produkce negativně koreluje s poškozením HK-2 buněk. NGAL je extracelulární protein produkovaný převážně v ledvinách. Nedávno byl použit jako slibný biomarker pro detekci renálního poškození in vivo. Přesto dosud nebyl použit jako biomarker in vitro, a ověření tohoto bylo hlavním cílem naší studie. cze
dc.peerreviewed yes eng
dc.publicationstatus published eng
dc.identifier.doi 10.1016/j.tiv.2016.11.012 eng
dc.relation.publisherversion http://www.sciencedirect.com/science/article/pii/S0887233316302430/pdfft?md5=ffce0ffeb7593cedc9fd3da7b17225cc&pid=1-s2.0-S0887233316302430-main.pdf eng
dc.identifier.wos 000393542600004 eng
dc.identifier.scopus 2-s2.0-84997783020
dc.identifier.obd 39879452 eng


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