Determination of Methotrexate at a Silver Solid Amalgam Electrode by Differential Pulse Voltammetry
Článekpeer-reviewedpostprintSoubory
Datum publikování
2016
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Abstrakt
An ultrasensitive voltammetric method is reported for the determination of methotrexate using a polished modified silver solid amalgam electrode. Electrochemical behavior of this compound was examined using cyclic, linear sweep, and differential pulse voltammetry. Methotrexate provided two reduction and one oxidation signal in acidic and neutral media. The first reduction peak (E-p=-350 millivolts vs. Ag|AgCl electrode) and supporting electrolyte of pH 5 was chosen for further experiments. Differential pulse voltammetry parameters were optimized in combination with adsorptive stripping and the applicability of the method was verified. A low limit of detection (1.5x10(-10)moles per liter), good repeatability of determination (relative standard deviation<3.5%, n=5), and good repeatability of measurements (relative standard deviation of 1.7% at 1.0x10(-7)mole per liter methotrexate and n=11) was obtained. The method was employed for the accurate determination of methotrexate in a chemotherapeutic preparation.
Rozsah stran
p. 122-134
ISSN
0003-2719
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Analytical Letters, volume 49, issue: 1
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
Chemotherapeutic drug, methotrexate, silver solid amalgam electrode, voltammetry, Chemoterapeutické léky, methotrexát, stříbrná pevná amalgámová elektroda, voltametrie