Digitální knihovnaUPCE
 

Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

ČlánekOtevřený přístuppeer-reviewedpostprint

Abstrakt

In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells?specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line.

Rozsah stran

p. 323-329

ISSN

0173-0835

Trvalý odkaz na tento záznam

Projekt

EC/FP7/228980/EU/Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells/CAMINEMS

Zdrojový dokument

Electrophoresis, volume 35, issue: 2-3

Vydavatelská verze

http://onlinelibrary.wiley.com/doi/10.1002/elps.201300196/full

Přístup k e-verzi

open access

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Klíčová slova

Biofunctionalization, Circulating tumor cells, EpCAM, Immunomagnetic separation, Poly(glycidyl methacrylate), Biofunkcionalizace, cirkulující nádorové buňky, EpCAM, imunomagnetická separace, poly(glycidyl methacrylát)

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