Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
ČlánekOtevřený přístuppeer-reviewedpostprintDatum publikování
2014
Vedoucí práce
Oponent
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Název svazku
Vydavatel
Wiley-VCH
Abstrakt
In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells?specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line.
Rozsah stran
p. 323-329
ISSN
0173-0835
Trvalý odkaz na tento záznam
Projekt
EC/FP7/228980/EU/Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells/CAMINEMS
Zdrojový dokument
Electrophoresis, volume 35, issue: 2-3
Vydavatelská verze
http://onlinelibrary.wiley.com/doi/10.1002/elps.201300196/full
Přístup k e-verzi
open access
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Klíčová slova
Biofunctionalization, Circulating tumor cells, EpCAM, Immunomagnetic separation, Poly(glycidyl methacrylate), Biofunkcionalizace, cirkulující nádorové buňky, EpCAM, imunomagnetická separace, poly(glycidyl methacrylát)