Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot
ČlánekOtevřený přístuppeer-reviewedpostprintDatum publikování
2013
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Pardubice
Abstrakt
The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation (IMS). The choice of methods for characterizing the antibodies is limited, especially with antibodies specific to surface cell structures. In this study, four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a newly adapted whole-cell dot blot technique were applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies from MyBiosource unambiguously demonstrated the highest immunoreactivity to Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such antibodies will be subsequently used for the preparation of an anti-Salmonella immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti-Salmonella poly- and monoclonal antibodies are also discussed.
Rozsah stran
p. 57-66
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
EC/FP7/317742/EU/Love wave fully integrated Lab-on-Chip platform for food pathogen detection/LOVE-FOOD
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19 (2013)
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
imunomagnetic separation of bacteria, imunosorbent, anti-Salmonella antibodies, whole-cell dot-blot, quality evaluation, imunomagnetická separace bakterií, imunosorbent, anti-Salmonella protilátky, dot-blot technika pro celé buňky, kvalitativní analýza