GC/MS and FAB/MS analysis of lipid molecules for taxonomic classification of microorganisms
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2005
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Pardubice
Abstrakt
The goal of this project was to identify the presence of microorganisms in the environment by using their gas chromatography/mass spectral (GC/MS) characteristics as biomarkers. A combination of GC/MS results and pattern recognition statistical evaluation were used to provide a mathematical model with defined parameters for different microorganisms, which allows the organisms to be classified as bacterial, fungal or viral nature. Results from fast atom bombardment mass spectral (FAB/MS) analysis showed differences in the complex lipid profiles for most of the analyzed microorganisms. A general screening method that can provide early detection of microorganisms would permit initiation of most therapeutics. Various drugs are used to treat bacterial, fungal or viral diseases and, when treatment is started early, there is a dramatic reduction in mortality. A methodology for detection and classification of microorganisms is described in this paper.
Rozsah stran
p. 131-146
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical technology. 10 (2004)
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
47333
Umístění tištěné verze
Univerzitní knihovna (studovna)
Přístup k tištěné verzi
Klíčová slova
plynová chromatografie, Hmotnostní spektrometrie, mikroorganismy, Klasifikace, Tuky