Complete fatty acid composition of selected Aspergillus species

Show simple item record Khailová, Ludmila Miketová, Petra Jelínek, David Schram, Karl H. Moore, Ida (Ki) Vytřasová, Jarmila 2009-03-12T16:11:25Z 2009-03-12T16:11:25Z 2006
dc.identifier Univerzitní knihovna (studovna) cze
dc.identifier.issn 1211-5541
dc.description.abstract The goal of this work was to perform a detailed analysis of fatty acids from Aspergillus species in a search for biomarkers to differentiate the spicies. In the course of this work, we isolated various classes of lipids (neutral lipids, glycolipids, phospholipids and lipopolysaccharides) from six Aspergillus species by chloroform/methanol extraction and multiple column fractionations. Further separation of structurally different fatty acids from phospholipids was also performed. The identities of isolated fatty acids from all lipid fractions were tentatively assigned using gas chromatography/mass spectrometry (GC/MS). The experimental methodology was found to be suitable for complete characterization of the fatty acids from various cellular sources. While the methods used in this work are standard techniques, the detal obtained in the fatty acid composition of each of the Aspergillus species is much greater than previously described. eng
dc.format p. 45-58 cze
dc.language.iso eng
dc.publisher Univerzita Pardubice cze
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical technology. 11(2005) eng
dc.subject mastné kyseliny cze
dc.subject Aspergillus spp. cze
dc.subject Aspergillus niger cze
dc.subject analytické metody cze
dc.subject plynová chromatografie cze
dc.subject Hmotnostní spektrometrie cze
dc.title Complete fatty acid composition of selected Aspergillus species eng
dc.type article cze
dc.identifier.signature 47333
dc.peerreviewed yes eng
dc.publicationstatus published eng

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