Comment on colorimetric monitoring of enzymatic hydrolysis of acetylthiocholine

dc.contributor.author	Komers, Karel
dc.contributor.author	Komersová, Alena
dc.contributor.author	Stratilová, Jana
dc.date.accessioned	2009-03-12T16:10:43Z
dc.date.available	2009-03-12T16:10:43Z
dc.date.issued	2004
dc.identifier	Univerzitní knihovna (studovna)	cze
dc.identifier.issn	1211-5541
dc.identifier.uri	http://hdl.handle.net/10195/32636
dc.description.abstract	The mechanism of the colorimetric reaction of thiocholine with Ellman's reagent has been studied. It was found that its mechanism is more complex than acknowledged so far and conforms to two consecutive competitive steps (2) and (3) with the condition for the rate constants k2k1. The applicabilityof this reaction to the very frequent on-line monitoringof the enzymatic hydrolysis of acetylthiocholine fortunately has not changed. The determined value of absorption coefficient of the measured yellow substance.	eng
dc.format	p. 89-96	cze
dc.language.iso	eng
dc.publisher	Univerzita Pardubice	cze
dc.relation.ispartof	Scientific papers of the University of Pardubice. Series A, Faculty of Chemical technology. 9 (2003)	eng
dc.rights	open access	eng
dc.title	Comment on colorimetric monitoring of enzymatic hydrolysis of acetylthiocholine	eng
dc.type	article	cze
dc.identifier.signature	47333
dc.peerreviewed	yes	eng
dc.publicationstatus	published	eng