Stability of enzymes immobilized in oriented way on magnetic and non-magnetic bead cellulose
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2002
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Pardubice
Abstrakt
The enzymes galactose oxidase and neuraminidase have been immobilized in an oriented way to hydrazide derivative of magnetic and non-magnetic macroporous bead cellulose. Binding was accomplished after the sugar components have been oxidatively activated by natrium periodate as the oxidating agent. The operational and storage stability of neuraminidase reactor was studied during four weeks and operational stability of galactose oxidase reactor during three weeks. The operationas stability of galactose oxidase immobilized to magnetic macroporous bead cellulose was excellent in the environment of Cu 2+ ions. Stability of immobilized neuraminidase to non-magnetic support had a similar course. The stability of neuraminidase immobilized to magnetic macroporous bead cellulose was decreasing rapidly.
Rozsah stran
p. 147-157
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical technology. 7 (2001)
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
47333
Umístění tištěné verze
Univerzitní knihovna (studovna)
Přístup k tištěné verzi
Klíčová slova
enzymy, stabilita, galaktosaoxidasa, imobilizace, oxidační reakce