Determination and profiling of fatty acids in dried breast milk spot

Abstract

A method was developed for the determination of fatty acids in dried breast milk spot and plasma using gas chromatography with mass spectrometry and flame ionization detection. The breast milk was sampled onto a collection paper treated with butylated hydroxytoluene. Fatty acids were derivatized with acetyl chloride in methanol to form fatty acids methyl esters. Our results indicate that the concentrations of fatty acids found in human milk can be used for assessing the intake and needs of infants.