OPTIMIZATION OF GRADIENT SEPARATION CONDITIONS IN TWO-DIMENSIONAL LIQUID PHASE SYSTEMS

Abstract

The gradient separation conditions in two-dimensional liquid chromatography are usually used for improvement of the separation efficiency and for better utilization of the separation space. In the work, procedures for optimization of both, gradient two-dimensional liquid chromatography and liquid chromatography coupled to capillary electrophoresis were developed. The in-laboratory build software for processing of two-dimensional data was extended with the optimization protocols.