Voltammetric Determination of Daminozide in Plant Material

Abstract

A new procedure for the determination of daminozide in plant material was proposed. The plant sample is dried in a vacuum oven at 50°C and 9 mbar pressure to constant weight. 96 % ethanol is used to isolate the analyte from the organic matrix under ultrasonication for 2 minutes. This is followed by filtration of the resulting suspension and isolation of the daminozide from the filtrate using Amberlite® IRC120 H catex in an H+ cycle. The analyte is displaced from the ion-exchange with a weakly alkaline NaCl solution at a concentration of 1 mol L1. The voltammetric analysis of daminozide is performed by anodic oxidation in 0.1 mol L1 chloroacetate buffer of pH 3.2 using a boron-doped diamond electrode.