Effect of immobilization methods on service life of tyrosinase biosensors developed for dopamine monitoring

Abstract

An effect of immobilization methods on service life of tyrosinase amperometric biosensors was studied where dopamine was selected as a model analyte. Three different immobilization methods were tested such as incorporation of enzyme into the electrode material, immobilization using perfluorosulfonic acid polymer Nafion® and immobilization of enzyme by covalent bonding with the assistance of cross-linking agent glutaraldehyde. All prepared amperometric tyrosinase biosensors were stored dry in refrigerator at 5°C. Finally, the effect of used immobilization was discussed in details.