Application of computerized accumulation of chromatograms in HPLC with various detection systems and with on- and off-line solid phase extraction sample enrichment

Abstract

Computerized accumulation of chromatograms acquired in several repeated runs may be used to enhance the signal-to-noise ratio and to decrease the detection limits in High Performance Liquid Chromatography (HPLC). The technique is feasible not only with short ("high-speed") columns, but also with conventional analytical columns, provided the separation is rapid. This approach can be applied in combination with UV, fluorescence and electrochemical detection systems and with the on- or off-line solid phase extraction (SPE) enrichment techniques. Computerized accumulation of chromatograms may further improve the detection limits achieved in HPLC with sensitive detection methods and the SPE sample enrichment.