Catecholase biomimetic copper(II) complex immobilization via Schiff's base formation onto the cross-linked polymers prepared by drop-casting

Abstract

In this article, a universal protocol is introduced dealing with covalent immobilization of catecholase biomimetic copper(II) complexes with free carbonyl group onto the primary amine-based polymeric substrates (chitosan and collagen, type II) cross-linked with glutar-aldehyde; both being enabled via Schiff's base formation. As a representative of biomimetic catalysts, [Cu2Ac2O(L)ClO4] consisting of a 2,6-bis(4-methyl piperazin-1-ylmethyl)- 4-formyl-phenoxy ligand (L) was selected. The resulting functionalized polymers formed at the surface of a screen-printed carbon electrode were characterized using scanning electron microscopy with energy dispersive X-ray analysis. As a result, the procedure presented herein could find use in the development of artificial biosensors.