Changes in concentrations of 5-hydroxymethylfurfural, carbohydrates, and ethanol during the mead production

Abstract

This work is focused on monitoring changes in the concentrations of 5-hydroxymethylfurfural, ethanol, and carbohydrates during the mead production using various analytical techniques, namely micellar electrokinetic chromatography with spectrophotometric detection, gas chromatography coupled to flame-ionization detector, and hydrophilic interaction liquid chromatography with refractive-index detector, respectively. In this study, production of three mead samples made by different Czech beekeepers has been monitored. The individual mead fractions were regularly sampled for two months of fermentation and always analysed with the aid of the optimised analytical approach.