PREDICTION OF GRADIENT RETENTION DATA FOR HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHIC SEPARATION OF NATIVE AND FLUORESCENTLY LABELED OLIGOSACCHARIDES

Abstract

In this study, we have investigated mixed-mode retention model and its extended version for predictions of retention times of native and fluorescently labelled oligosaccharides separated in hydrophilic interaction liquid chromatography (HILIC) in gradient elution mode. Native oligosaccharides (maltose to maltoheptaose) were derivatized using four reagents: 2-aminobenzoic acid, 2-aminobenzamide, 2-aminopyridine and 8-aminonaphthalene-1,3,6-trisulfonic acid. The separations were realized on the column packed with amide-type stationary phase in two different mobile phases consisted of acetonitrile and water with 0.1% acetic acid (v/v) and acetonitrile with 30 mM ammonium acetate.