The specifics of the general nurse profession and the demands placed on this profession are very diverse and burdensome. The adaptation process, working conditions, the performance of the profession within their competencies influencing the perception of one's own profession and are important factors for the satisfaction of general nurses with the performance of their profession. The authors present the main results of a questionnaire survey focused on the experience of Czech general nurses with the conditions for the performance of their profession in the Czech healthcare system. Specifically, the message focuses on the experience and satisfaction of nurses with the adaptation process, with the profession, with working conditions and with the setting of competencies. The questionnaire survey was conducted within the project, the group of respondents consisted of 2,479 general nurses working in Czech acute care facilities. Most respondents are satisfied with the performance of their profession. For the satisfaction it is important for the respondents to cooperate primarily with the nurses, but also with other staff, setting up the adaptation process in terms of length, the person of the trainer and specific processes. Respondents are the least satisfied with the setting and adherence to competencies. In the current shortage of general nurses in the Czech healthcare system, it is necessary to focus on the monitored factors and solve problems for both new and stabilized employees.