Abstrakt:
The study of effect of sugars and amino acids on the growth and survival of fourstrains
mixture of Arcoabcter butzleri in tryptic soy broth (TSB) with different salt
concentrations was carried out at 30 °C. A. butzleri was able to grow in TSB with
3.5 % NaCl whereas no viable counts were detected at 4.0 and 5.0 % after 0.5-day
incubation. Sucrose, trehalose, alanine, glutamine or proline at 0.25 mmol levels
were separately added to TSB with 3.5 % NaCl. The growth and survival
parameters were estimated according to Baranyi and Weibull model, respectively.
The growth of A. butzleri was affected by all the amino acids resulting in higher
growth rate, lag phase and maximum population density in medium with 3.5 %
NaCl. Slight increase in growth rate in the presence of sucrose and trehalose was
only observed. On the other hand, bacteria better survived when sugars were used,
increasing the first reduction time (δ). Comparing the results with TSB with
natural content of NaCl (0.5 %), only sucrose enhanced the growth of A. butzleri,
whereas both sugars and amino acids improved their survival resulting in
higher δ.
