Show simple item record
dc.contributor.author |
Havelek, Radim
|
cze |
dc.contributor.author |
Muthna, Darina |
cze |
dc.contributor.author |
Tomsik, Pavel |
cze |
dc.contributor.author |
Královec, Karel
|
cze |
dc.contributor.author |
Seifrtova, Martina
|
cze |
dc.contributor.author |
Cahlikova, Lucie
|
cze |
dc.contributor.author |
Hostalkova, Anna |
cze |
dc.contributor.author |
Safratova, Marcela
|
cze |
dc.contributor.author |
Perwein, Maria |
cze |
dc.contributor.author |
Cermakova, Eva |
cze |
dc.contributor.author |
Rezacova, Martina
|
cze |
dc.date.accessioned |
2018-02-27T03:40:01Z |
|
dc.date.available |
2018-02-27T03:40:01Z |
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dc.date.issued |
2017 |
eng |
dc.identifier.issn |
0009-2797 |
eng |
dc.identifier.uri |
https://hdl.handle.net/10195/70280 |
|
dc.description.abstract |
In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model. The results showed that haemanthamine, lycorine and haemanthidine exerted the highest antiproliferative activity. The mean growth percent (GP) value after a single-dose 10 mu M treatment was for haemanthamine 21%, for lycorine 21% and for haemanthidine 27% that of untreated control cells (100%). Furthermore, haemanthamine, lycorine and haemanthidine exhibited significant cytotoxicities against all the tested cell lines with individual IC50 values in the micromolar range. Dynamic real-time measures of impedance by xCELLigence indicated that these three compounds suppress cell proliferation after 10 h of treatment at a concentration of 10 mM or higher. Regrettably, in a follow-up in vivo antitumor activity study, haemanthamine showed no statistically significant reduction in the tumor size with no prolongation of survival time of Ehrlich tumor-bearing mice. Taken together, these results provide a new clue and guidance for exploiting Amaryllidaceae alkaloids as anticancer agents. |
eng |
dc.format |
p. 121-132 |
eng |
dc.language.iso |
eng |
eng |
dc.publisher |
Elsevier Ireland Ltd |
eng |
dc.relation.ispartof |
Chemico-biological Interactions, volume 275, issue: 25 September |
eng |
dc.rights |
Práce není přístupná |
eng |
dc.subject |
Antiproliferative activity |
eng |
dc.subject |
Amaryllidaceae |
eng |
dc.subject |
Alkaloids |
eng |
dc.subject |
In vitro |
eng |
dc.subject |
In vivo |
eng |
dc.subject |
Antiprolifeační aktivita |
cze |
dc.subject |
Amaryllidaceae |
cze |
dc.subject |
Alkaloidy |
cze |
dc.subject |
In vitro |
cze |
dc.subject |
In vivo |
cze |
dc.title |
Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice |
eng |
dc.title.alternative |
Protinádorový potenciál alkaloidů čeledi Amaryllidaceae testovaný na panelu lidských linií, pomocí analýzy buněk v reálném čase a na Ehrlichově nádoru myší |
cze |
dc.type |
article |
eng |
dc.description.abstract-translated |
V této studii byl testován protinádorový potenciál 22 alkaloidů čeledi Amaryllidaceae na panelu lidských linií, pomocí analýzy buněk v reálném čase a na Ehrlichově nádoru myší. |
cze |
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
published |
eng |
dc.identifier.doi |
10.1016/j.cbi.2017.07.018 |
eng |
dc.relation.publisherversion |
http://www.sciencedirect.com/science/article/pii/S0009279717306713?via%3Dihub |
eng |
dc.identifier.wos |
000410631400012 |
eng |
dc.identifier.scopus |
2-s2.0-85026742575 |
|
dc.identifier.obd |
39879248 |
eng |
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