A study on the characterization of kinetics of the particle growth of electrically charged silver nanoparticles at concentrations between 25 and 50 umol/L was performed. Analysis and time-dependent fitting of the diameters of the silver colloidal agglomerates was characterized using atomic force microscopy and optical techniques. The toxicity of silver agglomerates with diameters 400-500 nm on fish embryos was characterized.