Determination of the polyphenolic content of a Capsicum annuum L. extract by liquid chromatography coupled to photodiode array and mass spectrometry detection and evaluation of its biological activity

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dc.contributor.author Mokhtar Meriem
dc.contributor.author Soukup Jan
dc.contributor.author Donato Paola
dc.contributor.author Cacciola Francesco
dc.contributor.author Riazi Ali
dc.contributor.author Jandera Pavel
dc.contributor.author Dugo Paola
dc.contributor.author Mondello Luigi
dc.date.accessioned 2016-11-14T08:19:21Z
dc.date.available 2016-11-14T08:19:21Z
dc.date.issued 2015
dc.identifier.issn 1615-9306
dc.identifier.uri http://hdl.handle.net/10195/66431
dc.description.abstract The present study was aimed to investigate the polyphenolic profile of a pepper (Capsicum annuum L.) extract from Algeria and evaluate its biological activity. The total polyphenol content of the extract was determined as 1.373 mg of gallic acid equivalents (±0.0046), whereas the flavonoids were determined as 0.098 mg of quercetin (±0.0015). The determination of the complete polyphenolic profile of the extract was achieved by liquid chromatography with an RP-amide column in combination with photodiode array and mass spectrometry detection through an electrospray ionization interface. A total of 18 compounds were identified, of which five were reported for the first time in the sample tested. Quercetin rhamnoside was the most abundant compound (82.6 g/g of fresh pepper) followed by quercetin glucoside (19.86 g/g). The antioxidant activity and antimicrobial effects were also determined. For the antimicrobial tests assessed against Gram-positive and Gram-negative bacteria, kaempferol showed the strongest inhibitory effect followed by quercetin and caffeic acids. In the study of the cytotoxicity of the extract, the cancer cells (U937) were more affected than the normal cells (peripheral blood mononucleated cells), with more than 62% inhibition at the highest concentration. eng
dc.format p. 171-178 eng
dc.language.iso eng
dc.publisher Wiley-VCH eng
dc.relation.ispartof Journal of Separation Science, volume 38, issue: 2 eng
dc.rights Pouze v rámci univerzity eng
dc.subject Antimicrobial activity eng
dc.subject Antioxidant activity eng
dc.subject Capsicum annuum L eng
dc.subject Liquid chromatography eng
dc.subject Polyphenols eng
dc.subject antimikrobiální aktivita cze
dc.subject antioxidační aktivita cze
dc.subject Capsicum annum cze
dc.subject kapalinová chromatografie cze
dc.subject polyfenoly cze
dc.title Determination of the polyphenolic content of a Capsicum annuum L. extract by liquid chromatography coupled to photodiode array and mass spectrometry detection and evaluation of its biological activity eng
dc.title.alternative Stanovení polyfenolických látek a biologické aktivity v Capsicum annuum L. pomocí kapalinové chromatografie spojené s fotodiodovým polem a hmotnostní spektrometrií cze
dc.type article eng
dc.description.abstract-translated Uvedená studie pojednává o stanovení polyfenolů v zelených paprikách pocházejících z Alžírska. Celkový obsah polyfenolů byl 1,373 mg ekvivalentu ke kyselině gallové a 0,098 mg quercetinu. Stanovení úplného polyfenolického profilu v extraktu paprik bylo provedeno pomocí kapalinové chromatografies RP-amidovou kolonou s UV a hmotnostní detekcí s ionizací elektrosprejem. V paprikách jsme identifikovali celkem 18 látek, z nichž pět bylo objeveno poprvé. cze
dc.peerreviewed yes eng
dc.publicationstatus postprint eng
dc.identifier.wos 000348516600001
dc.identifier.obd 39872103


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