dc.contributor.author |
Šálek Petr
|
|
dc.contributor.author |
Korecká Lucie
|
|
dc.contributor.author |
Horák Daniel
|
|
dc.contributor.author |
Petrovský Eduard
|
|
dc.contributor.author |
Kovářová Jana
|
|
dc.contributor.author |
Metelka Radovan
|
|
dc.contributor.author |
Čadková Michaela
|
|
dc.contributor.author |
Bílková Zuzana
|
|
dc.date.accessioned |
2016-11-14T08:18:58Z |
|
dc.date.available |
2016-11-14T08:18:58Z |
|
dc.date.issued |
2011 |
|
dc.identifier.issn |
0959-9428 |
|
dc.identifier.uri |
http://hdl.handle.net/10195/66358 |
|
dc.description.abstract |
Poly(styrene-co-divinylbenzene) microspheres of narrow size distribution were prepared by (2-hydroxypropyl) cellulose-stabilized dispersion copolymerization of styrene and divinylbenzene in a 2-methoxyethanol/ethanol mixture under continuous addition of divinylbenzene. The copolymerization was initiated with dibenzoyl peroxide. The obtained microspheres were chloromethylated using several chloromethylation agents and then hypercrosslinked. Their porous structure was analyzed by nitrogen adsorption and mercury porosimetry. Superparamagnetic iron oxide nanoparticles were precipitated within the pores of microspheres from Fe(II) and Fe(III) chloride solution. The Fe content in the microspheres was determined by carbon analysis, atomic absorption spectroscopy and thermogravimetric analysis. Magnetic properties of the microspheres were characterized by magnetization curves and the temperature dependence of magnetic susceptibility. Finally, sulfo groups were introduced into the microspheres to prepare an immunomagnetic electrochemical biosensor for protein detection with ovalbumin as a model substance. |
eng |
dc.format |
p. 14783-14792 |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Royal Society of Chemistry |
eng |
dc.relation.ispartof |
Journal of Materials Chemistry, volume 21, issue: 38 |
eng |
dc.rights |
Pouze v rámci univerzity |
eng |
dc.subject |
poly(glycidyl methacrylate) microspheres |
eng |
dc.subject |
dispersion polymerization |
eng |
dc.subject |
magnetic particles |
eng |
dc.subject |
miniemulsion polymerization |
eng |
dc.subject |
silica microspheres |
eng |
dc.subject |
size distribution |
eng |
dc.subject |
nanoparticles |
eng |
dc.subject |
beads |
eng |
dc.subject |
monodisperse |
eng |
dc.subject |
polyglycidyl methacrylátové mikročástice |
cze |
dc.subject |
disperzní polymerace |
cze |
dc.subject |
magnetické částice |
cze |
dc.subject |
proteiny |
cze |
dc.subject |
elektrochemická detekce |
cze |
dc.title |
Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres forelectrochemical detection of proteins |
eng |
dc.title.alternative |
Imunomagnetické solfonované hypersíťované polystyrenové mikročástice pro elektrochemickou detekci proteinů |
cze |
dc.type |
article |
eng |
dc.description.abstract-translated |
Polystyrenové mikročástice s úzkou distribucí velikosti byly připraveny (2-hydroxypropyl)celulozou stabilizovanou disperzní kopolymerací styrenu a divinylbenzenu. Získané mikročástice byly chlormethylovány různými činidly a následně hypersíťovány. Jejich porézní struktura byla analyzována. Superparamagnetické nanočástice tvořené oxidy železa byly precipitovány v pórech mikročástic y roztoku chloridů železa. Obsah železa byl určen uhlíkovou analýzou, AAS a termogravinetricky. Magnetické vlastnosti mikročástic byly charakterizovány. Nakonec byly na částice zavedeny sulfo skupiny a využity pro přípravu immunomagnetického elektrochemického biosenzoru pro detekci proteinů s využitím ovalbuminu jako modelového proteinu. |
cze |
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
postprint |
eng |
dc.identifier.wos |
000295101000065 |
|
dc.identifier.obd |
39856356 |
|