PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Show simple item record Kučerová, Jana Svobodová, Zuzana Knotek, Petr Palarčík, Jiří Vlček, Milan Kincl, Miloslav Horák, Daniel Autebert, Julien Viovy, Jean-Louis Bílková, Zuzana 2016-04-21T13:49:35Z 2016-04-21T13:49:35Z 2014
dc.identifier.issn 0928-4931
dc.description.abstract In this study, magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly(ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells while decreasing adhesion of particles to the walls of a microfluidic devices from poly(dimethylsiloxane) (PDMS) and cyclic olefin copolymer (COC). Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, and bioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres. Both neat and PEGylated microspheres were then incubated with the inert protein bovine serum albumin or cells to evaluate the rate of nonspecific adsorption (NSA). PEG with Mr of 30,000 Da was responsible for 45% reduction in NSA of proteins and 74% for cells compared to neat particles. The microspheres' behavior in PDMS and COC microchannels was then evaluated. Aggregation and adhesion of PEGylated microspheres significantly decreased compared to neat particles. Finally, the model enzyme horseradish peroxidase was immobilized on the microspheres through the heterobifunctional PEG chain. The possibility for subsequent covalent coupling of the ligand of interest was confirmed. Such PEGylated microparticles can be efficiently used in PDMS microchips as a carrier for bioaffinity separation or of enzyme for catalysis. eng
dc.format p. 308-315 eng
dc.language.iso eng
dc.publisher Elsevier cze
dc.relation.ispartof Materials Science and Engineering: C. 2014, vol. 40 eng
dc.rights open access eng
dc.rights Attribution-NonCommercial-NoDerivs 4.0 Czech Republic *
dc.rights.uri *
dc.subject PEGylation eng
dc.subject magnetic microspheres eng
dc.subject microfluidics eng
dc.subject nonspecific adsorption eng
dc.title PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays eng
dc.type Article eng
dc.peerreviewed yes eng
dc.publicationstatus postprint eng
dc.identifier.doi 10.1016/j.msec.2014.04.011
dc.project.ID EC/FP7/317742/EU/Love wave fully integrated Lab-on-Chip platform for food pathogen detection/LOVE-FOOD
dc.project.ID EC/FP7/246513/EU/Nanosystems for the early Diagnosis of Neurodegenerative diseases/NaDiNe
dc.project.ID EC/FP7/228980/EU/Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells/CAMINEMS
dc.identifier.wos WOS:000338388400041
dc.identifier.scopus 2-s2.0-84899637658

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