Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot

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dc.contributor.author Kučerová, Jana
dc.contributor.author Holubová, Lucie
dc.contributor.author Jankovičová, Barbora
dc.contributor.author Dvořáková, Veronika
dc.contributor.author Moťková, Petra
dc.contributor.author Bílková, Zuzana
dc.date.accessioned 2016-04-12T15:12:51Z
dc.date.available 2016-04-12T15:12:51Z
dc.date.issued 2013
dc.identifier.issn 1211-5541
dc.identifier.uri http://hdl.handle.net/10195/61976
dc.description.abstract The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation (IMS). The choice of methods for characterizing the antibodies is limited, especially with antibodies specific to surface cell structures. In this study, four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a newly adapted whole-cell dot blot technique were applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies from MyBiosource unambiguously demonstrated the highest immunoreactivity to Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such antibodies will be subsequently used for the preparation of an anti-Salmonella immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti-Salmonella poly- and monoclonal antibodies are also discussed. eng
dc.format p. 57-66 eng
dc.language.iso eng
dc.publisher Univerzita Pardubice cze
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19 (2013) eng
dc.rights open access eng
dc.subject imunomagnetic separation of bacteria eng
dc.subject imunosorbent eng
dc.subject anti-Salmonella antibodies eng
dc.subject whole-cell dot-blot eng
dc.subject quality evaluation eng
dc.subject imunomagnetická separace bakterií cze
dc.subject imunosorbent cze
dc.subject anti-Salmonella protilátky cze
dc.subject dot-blot technika pro celé buňky cze
dc.subject kvalitativní analýza cze
dc.title Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot eng
dc.title.alternative Kvalitativní hodnocení komerčně dostupných anti-salmonelových protilátek pro imunomagnetickou separaci pomocí dot-blot analýzy celých buněk cze
dc.type Article cze
dc.description.abstract-translated Klíčovým krokem při přípravě imunosorbentu pro efektivní imunomagnetickou separaci buněk je výběr protilátek s požadovanou specifitou, imunoreaktivitou a čistotou. Jejich charakterizace z pohledu těchto vlastností je obtížná, zejména pokud se jedná o protilátky proti povrchových buněčným strukturám. Tato studie se zabývá kvalitativním hodnocení čtyř komerčně dostupných anti-salmonelových protilátek z pohledu jejich čistoty a imunoreaktivity na buňky bakterie Salmonella Typhimurium. Ke kvalitativní analýze vybraných protilátek byla použita tradiční metoda SDS-PAGE a nově adaptovaná dot-blot technika pro celé buňky. Výsledky této studie ukazují nejvyšší imunoreaktivitu monoklonálních anti-Salmonella protilátek od firmy MyBiosource. Zkřížená reaktivita s G- bakteriemi Escherichia coli a Citrobacter braakii nebyla pozorována. cze
dc.peerreviewed yes eng
dc.publicationstatus postprint eng
dc.project.ID EC/FP7/317742/EU/Love wave fully integrated Lab-on-Chip platform for food pathogen detection/LOVE-FOOD eng

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