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dc.contributor.author |
Soukup, Jan |
|
dc.contributor.author |
Janás, Petr |
|
dc.contributor.author |
Jandera, Pavel |
|
dc.date.accessioned |
2015-02-10T17:32:04Z |
|
dc.date.available |
2015-02-10T17:32:04Z |
|
dc.date.issued |
2014 |
|
dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
http://hdl.handle.net/10195/58646 |
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dc.description.abstract |
We studied the influence of mobile phase composition on the retention of phenolic acids and flavonoid compounds on five different hydrosilated silica-based stationary phases in buffered aqueous acetonitrile. Cogent UDC cholesterolTM, Cogent bidentate C18TM and Cogent Phenyl hydrideTM columns show significant dual reversed-phase/normal-phase retention behavior, while Cogent Diamond hydrideTM and Cogent Silica-CTM columns provide very low retention in the reversed-phase mode. The effect of the aqueous acetate buffer concentration on retention factors of phenolic acids and flavonoid compounds over the full mobile phase composition range, including both aqueous normal-phase (ANP) and reversed-phase (RP) mechanisms, can be described by a four-parameter equation for dual-retention mechanism. At increasing temperature, the retention factors and peak widths decrease in the aqueous normal-phase range. In agreement with van’t Hoff model, linear ln k versus 1/T plots were observed, showing a single retention mechanism. From among the stationary phases tested, Cogent UDC cholesterolTM column has high temperature stability (up to 100/C) and provides most selective and efficient separations of flavones in the ANP mode and Cogent Diamond hydrideTM is the most selective and effective for separation of phenolic acids. |
eng |
dc.format |
p. 35-68 |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Univerzita Pardubice |
cze |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 20 (2014) |
eng |
dc.rights |
pouze v rámci univerzity |
cze |
dc.subject |
hydrosilated silica |
eng |
dc.subject |
HILIC |
eng |
dc.subject |
antioxidants |
eng |
dc.subject |
hydrosilovaný silikagel |
cze |
dc.subject |
antioxidanty |
cze |
dc.title |
Utilization of hydrosilated silica-based stationary phases for separation of antioxidants |
cze |
dc.title.alternative |
Využití hydrosilovaných stacionárních fází pro separaci antioxidantů |
cze |
dc.type |
Article |
cze |
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
published |
eng |
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