Zobrazit minimální záznam
dc.contributor.author |
Souza Gil, Eric de |
|
dc.contributor.author |
Rezende, Stefani Garcia |
|
dc.contributor.author |
Júnior, Eli José Miranda Ribeiro |
|
dc.contributor.author |
Barcelos, Hernane Toledo |
|
dc.contributor.author |
Scalize, Paulo Sérgio |
|
dc.contributor.author |
Santiago, Mariangela Fontes |
|
dc.contributor.author |
Quintino, Michelle Pereira |
|
dc.contributor.author |
Somerset, Vernon Sydwill |
|
dc.date.accessioned |
2014-10-21T07:32:25Z |
|
dc.date.available |
2014-10-21T07:32:25Z |
|
dc.date.issued |
2014 |
|
dc.identifier.isbn |
978-80-7395-782-7 (print) |
|
dc.identifier.isbn |
978-80-7395-783-4 (pdf) |
|
dc.identifier.uri |
http://hdl.handle.net/10195/58400 |
|
dc.description.abstract |
Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase
source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV s-1 and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L-1, with a detection limit of 30 nmol L-1. This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. |
eng |
dc.format |
p. 227-240 |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Univerzita Pardubice |
|
dc.relation.ispartof |
Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2013/2014, Volume 8. |
eng |
dc.rights |
open access |
eng |
dc.subject |
biosensor |
eng |
dc.subject |
laccase |
eng |
dc.subject |
phenolic micropollutants |
eng |
dc.subject |
catechol |
eng |
dc.subject |
Pycnoporus sanguineus fungi |
eng |
dc.subject |
functionalised silica |
eng |
dc.title |
Development of a laccase biosensor for determination of Phenolic micropollutants in surface waters |
eng |
dc.type |
Article |
|
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
published |
eng |
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