Comprehensive profiling of APTS-Labeled N-Glycans using capillary zone electrophoresis/laser induced fluorescence

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dc.contributor.author Staněk, Václav
dc.contributor.author Starkey, Jason A.
dc.contributor.author Novotny, Milos V.
dc.contributor.author Jandera, Pavel
dc.date.accessioned 2014-10-09T08:39:29Z
dc.date.available 2014-10-09T08:39:29Z
dc.date.issued 2012
dc.identifier.isbn 978-80-7395-563-2 (printed)
dc.identifier.isbn 978-80-7395-564-9 (on-line)
dc.identifier.uri http://hdl.handle.net/10195/58372
dc.description.abstract In this article, a method for the analysis of N-linked glycans derived from glycoproteins is described. Complex mixtures of APTS-labeled neutral and acidic N-glycans were resolved using capillary zone electrophoresis coupled with laser-induced fluorescence detector. All separations were carried out on a polyacrylamide coated capillary with reduced electroosmotic flow. N-glycan pools released from several standard glycoproteins were analyzed in order to generate a migration time database used for the identification of N-glycans released from more complicated samples. In addition, enzymatic digestions using specific and non-specific exoglycosidases were used to positively identify glycan isomeric structures. eng
dc.format p. 443-458 eng
dc.language.iso eng
dc.publisher Univerzita Pardubice
dc.relation.ispartof Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2012, Volume 7. eng
dc.rights open access eng
dc.subject capillary zone electrophoresis eng
dc.subject laser induced fluorescence eng
dc.subject glycoproteins eng
dc.subject N-glycans eng
dc.subject glycan profiling eng
dc.title Comprehensive profiling of APTS-Labeled N-Glycans using capillary zone electrophoresis/laser induced fluorescence eng
dc.type Article
dc.peerreviewed yes eng
dc.publicationstatus published eng


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