Comprehensive profiling of APTS-Labeled N-Glycans using capillary zone electrophoresis/laser induced fluorescence
ČlánekStatus neznámýpeer-reviewedpublishedDatum publikování
2012
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Vydavatel
Univerzita Pardubice
Abstrakt
In this article, a method for the analysis of N-linked glycans derived from glycoproteins is described. Complex mixtures of APTS-labeled neutral and acidic N-glycans were resolved using capillary zone electrophoresis coupled with laser-induced fluorescence detector. All separations were carried out on a polyacrylamide coated capillary with reduced electroosmotic flow. N-glycan pools released from several standard glycoproteins were analyzed in order to generate a migration time database used for the identification of N-glycans released from more complicated samples. In addition, enzymatic digestions using specific and non-specific exoglycosidases were used to positively identify glycan isomeric structures.
Rozsah stran
p. 443-458
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Zdrojový dokument
Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2012, Volume 7.
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open access
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ISBN
978-80-7395-563-2 (printed)
978-80-7395-564-9 (on-line)
978-80-7395-564-9 (on-line)
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Klíčová slova
capillary zone electrophoresis, laser induced fluorescence, glycoproteins, N-glycans, glycan profiling