Zobrazit minimální záznam
dc.contributor.author |
Staněk, Václav |
|
dc.contributor.author |
Starkey, Jason A. |
|
dc.contributor.author |
Novotny, Milos V. |
|
dc.contributor.author |
Jandera, Pavel |
|
dc.date.accessioned |
2014-10-09T08:39:29Z |
|
dc.date.available |
2014-10-09T08:39:29Z |
|
dc.date.issued |
2012 |
|
dc.identifier.isbn |
978-80-7395-563-2 (printed) |
|
dc.identifier.isbn |
978-80-7395-564-9 (on-line) |
|
dc.identifier.uri |
http://hdl.handle.net/10195/58372 |
|
dc.description.abstract |
In this article, a method for the analysis of N-linked glycans derived from glycoproteins is described. Complex mixtures of APTS-labeled neutral and acidic N-glycans were resolved using capillary zone electrophoresis coupled with laser-induced fluorescence detector. All separations were carried out on a polyacrylamide coated capillary with reduced electroosmotic flow. N-glycan pools released from several standard glycoproteins were analyzed in order to generate a migration time database used for the identification of N-glycans released from more complicated samples. In addition, enzymatic digestions using specific and non-specific exoglycosidases were used to positively identify glycan isomeric structures. |
eng |
dc.format |
p. 443-458 |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Univerzita Pardubice |
|
dc.relation.ispartof |
Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2012, Volume 7. |
eng |
dc.rights |
open access |
eng |
dc.subject |
capillary zone electrophoresis |
eng |
dc.subject |
laser induced fluorescence |
eng |
dc.subject |
glycoproteins |
eng |
dc.subject |
N-glycans |
eng |
dc.subject |
glycan profiling |
eng |
dc.title |
Comprehensive profiling of APTS-Labeled N-Glycans using capillary zone electrophoresis/laser induced fluorescence |
eng |
dc.type |
Article |
|
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
published |
eng |
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