Determination of coproporphyrin I and III in human urine using HPLC with fluorescence detection
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2010
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Pardubice
Abstrakt
The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the
intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 :g l–1, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0-51.6 :g l–1
88 Kanďár R. et al./Sci. Pap. Univ. Pardubice Ser. A 16 (2010) 87–98(0.59-3.20 :g mmol–1 creatinine) and 14.9-65.4 :g L-1 (0.98-8.21 :g mmol–1 creatinine). The presented method is inexpensive and suitable for screening of
porphyrias.
Rozsah stran
p. 87-98
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 16 (2010)
Vydavatelská verze
Přístup k e-verzi
Název akce
ISBN
978-80-7395-386-7 (Online)
978-80-7395-368-3 (Print)
978-80-7395-368-3 (Print)
Studijní obor
Studijní program
Signatura tištěné verze
47333-16
Umístění tištěné verze
Univerzitní knihovna (studovna)
Přístup k tištěné verzi
Klíčová slova
porphyrins, HPLC method, coproporphyrin I, coproporphyrin III