Application of electrochemical sensors for determination of anticholinesterase activity and immobilization of enzyme

Abstract

Cholinesterases are important enzymes, which are responsible for the rapid elimination of acetyclholine after its releasing at cholinergic synapses. High activity of cholinesterases is present in patients with Alzheimer´s disease. Therefore, testing of new inhibitors of cholinesterases are important. For determination of the inhibition efficiency several methods can be used. In our case, the anticholinesterase activity expressed by IC50 of five carbamate compounds were tested using screen-printed electrodes and square-wave voltammetry. The next part of our work was focused on testing of two types of immobilization of enzyme on the surface of screen-printed electrodes. Kinetic parameters (Km, Vm) were determined for immobilized and soluted enzyme. Obtained results were disscused.