New materials and techniques for separation and analysis of clinically important proteins

Abstract

This doctoral thesis deals with analysis of peptides and proteins using several analytical methods, mainly chromatography and mass spectrometry with following bioinformatic data processing. For the purpose of analysis of these important biomolecules, various techniques have been developed mainly for their targeted isolation from complex samples. These techniques also utilize newly introduced materials that bring a broad range of benefits such as high selectivity. Immobilization of various enzymes on selected micro- and nanomaterials is dealt with in another part of this thesis. Such created carriers with immobilized enzymes are subsequently utilized either for protein phosphorylation/dephosphorylation or for digestion of proteins.